SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Satar,Rukhsana; Jafri,Mohammad Alam; Rasool,Mahmood; Ansari,Shakeel Ahmed.

ABSTRACT This review article highlights the role of glutaraldehyde as a matrix activator/stabilizer in imparting higher operational and thermal stability to β-galactosidase (βG) for biotechnological applications. Glutaraldehyde has been used extensively as a crosslinking agent as well as for functionalization of matrices to immobilize β-galactosidase. Immobilized β-galactosidase systems (IβGS) obtained as a result of glutaraldehyde treatment has been employed to hydrolyze whey and milk lactose in batch reactors, continuous packed-bed and fluidized bed reactors under various operational conditions. Moreover, these IβGS have also been utilized for the production of galactooligosaccharides in food, dairy and fermentation industries. It was observed that...

Simultaneous biodegradation of phenol and cyanide present in coke-oven effluent using immobilized Pseudomonas putida and Pseudomonas stutzeri

Palavras-chave: Β-galactosidase; Crosslinking; Glutaraldehyde; Immobilization; Stability.

Ano: 2017

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100447

Singh,Utkarsh; Arora,Naveen Kumar; Sachan,Preeti.

ABSTRACT Discharge of coke-oven wastewater to the environment may cause severe contamination to it and also threaten the flora and fauna, including human beings. Hence before dumping it is necessary to treat this dangerous effluent in order to minimize the damage to the environment. Conventional technologies have inherent drawbacks however, biological treatment is an advantageous alternative method. In the present study, bacteria were isolated from the soil collected from the sites contaminated by coke-oven effluent rich in phenol and cyanide. Nucleotides sequence alignment and phylogenetic analysis showed the identity of the selected phenol and cyanide degrading isolates NAUN-16 and NAUN-1B as Pseudomonas putida and Pseudomonas stutzeri, respectively....

Stability of free and immobilized Lactobacillus acidophilus and Bifidobacterium lactis in acidified milk and of immobilized B. lactis in yoghurt

Palavras-chave: 16S rRNA; Biodegradation; Immobilization; Pseudomonas; Saw dust.

Ano: 2018

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100038

Grosso,Carlos Raimundo Ferreira; Fávaro-Trindade,Carmen Sílvia.

This study evaluated the stability of Bifidobacterium lactis (Bb-12) and of Lactobacillus acidophilus (La-05) both free and immobilized in calcium alginate, in milk and in acidified milk (pH 5.0, 4.4 and 3.8). The stability of immobilized B. lactis in yoghurt (fermented to pH 4.2), during 28 days of refrigerated storage was also evaluated. The efficiency of two culture media (modified MRS agar and Reinforced Clostridial Agar plus Prussian Blue) for counting of B. lactis in yoghurt was determined. Lee's agar was used to count Streptococcus thermophilus and Lactobacillus delbrueckii ssp. bulgaricus when B. lactis were counted in the MRS medium. B. lactis and L. acidophilus in both free and immobilized forms presented satisfactory rates of survival in milk...

Statistical Modelling and Optimization of Fermentation Medium for Lincomycin Production by Streptomyces lincolnensis Immobilized Cells

Palavras-chave: Probiotics; Immobilization; Alginate; Lactobacillus acidophilus; Bifidobacterium lactis; Yoghurt; Milk; Lactobacillus bulgaricus.

Ano: 2004

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100025

Abdelwahed,Nayera A.M.; Gomaa,Eman Zakaria; Hassan,Amira A..

ABSTRACT Response surface methodology was used to optimize lincomycin production by Streptomyces lincolnensis NRRL ISP-5355 in submerged fermentation. Screening of fermentation medium components to find their relative effect on lincomycin production was done using Plackett-Burman design. Malt extract, dextrin, soluble starch and (NH4)2SO4 were the most significant nutrient influenced on lincomycin production. Central composite design was applied to determine optimal concentrations of these factors and the effect of their mutual interactions. The interaction between soluble starch and (NH4)2SO4 was found to enhance the production, whereas malt extract and dextrin exhibited an influence independent from the other two factors. Using this statistical...

Sulfated fucan as support for antibiotic immobilization

Palavras-chave: Optimization; Lincomycin; Streptomyces lincolnensis; Response surface methodology; Immobilization.

Ano: 2017

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100405

BJMBR

Araújo,P.M.; Oliveira,G.B.; Córdula,C.R.; Leite,E.L.; Carvalho Jr.,L.B.; Silva,M.P.C..

Xylofucoglucuronan from Spatoglossum schröederi algae was tested as a support for antibiotic immobilization. The polysaccharide (20 mg in 6 ml) was first activated using carbodiimide, 1-ethyl-3-(3-dimethylamino-propyl)carbodiimide methiodide (20 mg in 2 ml), under stirring for 1 h at 25ºC and pH from 4.5 to 5.0. After adjusting the pH to 8.0, either gentamicin or amikacin (62.5 mg in 1.25 ml) was then immobilized on this chemically modified polysaccharide with shaking for 24 h in a cold room. Infrared spectra of the activated carbodiimide xylofucoglucuronan showed two bands to carbonyl (C = O at 1647.9 and 1700.7 cm-1) and to amide (C<FONT FACE=Symbol>Ý</FONT>-NH2) groups (1662.8 and 1714.0 cm-1). Microbial characterization of the derivatives...

Tipo: Info:eu-repo/semantics/other

Palavras-chave: Sulfated fucan; Immobilization; Antibiotics; Gentamicin; Amikacin.

Ano: 2004

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000300002

Synthesis of structured triacylglycerols enriched in n-3 fatty acids by immobilized microbial lipase

Araújo,Maria Elisa Melo Branco de; Campos,Paula Renata Bueno; Alberto,Thiago Grando; Contesini,Fabiano Jares; Carvalho,Patrícia de Oliveira.

Abstract The search for new biocatalysts has aroused great interest due to the variety of micro-organisms and their role as enzyme producers. Native lipases from Aspergillus niger and Rhizopus javanicus were used to enrich the n-3 long-chain polyunsaturated fatty acids content in the triacylglycerols of soybean oil by acidolysis with free fatty acids from sardine oil in solvent-free media. For the immobilization process, the best lipase/support ratios were 1:3 (w/w) for Aspergillus niger lipase and 1:5 (w/w) for Rhizopus javanicus lipase using Amberlite MB-1. Both lipases maintained constant activity for 6 months at 4 °C. Reaction time, sardine-free fatty acids:soybean oil mole ratio and initial water content of the lipase were investigated to determine...

The effect of a low dose of clenbuterol on rat soleus muscle submitted to joint immobilization

Palavras-chave: Lipase; Immobilization; Soybean oil; Polyunsaturated fatty acids.

Ano: 2016

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000401006

BJMBR

Cancelliero,K.M.; Durigan,J.L.Q.; Vieira,R.P.; Silva,C.A.; Polacow,M.L.O..

The aim of the present study was to evaluate the effect of joint immobilization on morphometric parameters and glycogen content of soleus muscle treated with clenbuterol. Male Wistar (3-4 months old) rats were divided into 4 groups (N = 6 for each group): control, clenbuterol, immobilized, and immobilized treated with clenbuterol. Immobilization was performed with acrylic resin orthoses and 10 µg/kg body weight clenbuterol was administered subcutaneously for 7 days. The following parameters were measured the next day on soleus muscle: weight, glycogen content, cross-sectional area, and connective tissue content. The clenbuterol group showed an increase in glycogen (81.6%, 0.38 ± 0.09 vs 0.69 ± 0.06 mg/100 g; P < 0.05) without alteration in weight,...

The production of sorbitol by permeabilized and immobilized cells of Z. mobilis in sucrose

Palavras-chave: Immobilization; Clenbuterol; Connective tissue; Glycogen content; Morphometry; Rat soleus muscle.

Ano: 2008

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001200003

Vignoli,Josiane Alessandra; Celligoi,Maria Antonia Colabone; Silva,Rui Sérgio Ferreira da; Barros,Márcio de.

The production of sorbitol by permeabilized and immobilized cells of Zymomonas mobilis in Luffa cylindrica was investigated in sucrose medium. A full 2³ factorial design was used to verify the influence of each factor and its interactions. The cell permeabilization showed a significant and negative effect upon the production of sorbitol, while the time of cultivation and the immobilization process were significant and positive. The results demonstrated that the cell immobilization and the time of cultivation of 36 h presented higher production of sorbitol.

The whole-cell immobilization of D-hydantoinase-engineered Escherichia coli for D-CpHPG biosynthesis

Palavras-chave: Permeabilization; Immobilization; Sucrose; Z. mobilis.

Ano: 2006

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500020

Electron. J. Biotechnol.

Jin,Yuan-yuan; Li,Ya-dong; Sun,Wan; Fan,Shuai; Feng,Xiao-zhou; Wang,Kang-you; He,Wei-qing; Yang,Zhao-yong.

Background: D-Hydroxyphenylglycine is considered to be an important chiral molecular building-block of antibiotic reagents such as pesticides, and β-lactam antibiotics. The process of its production is catalyzed by D-hydantoinase and D-carbamoylase in a two-step enzyme reaction. How to enhance the catalytic potential of the two enzymes is valuable for industrial application. In this investigation, an Escherichia coli strain genetically engineered with D-hydantoinase was immobilized by calcium alginate with certain adjuncts to evaluate the optimal condition for the biosynthesis of D-carbamoyl-p-hydroxyphenylglycine (D-CpHPG), the compound further be converted to D-hydroxyphenylglycine (D-HPG) by carbamoylase. Results: The optimal medium to produce...

Tipo: Journal article

Palavras-chave: Calcium alginate; D-Carbamoyl-p-hydroxyphenylglycine; D-Hydantoinase; D-Hydroxyphenylglycine; Immobilization; Whole cell.

Ano: 2016

URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000300004

Thiocyanate degradation by pure and mixed cultures of microorganisms

Souza-Fagundes,Elaine M.; Rosa,Luiz H.; Gomes,Newton C.M.; Santos,Mércia H.; Pimentel,Patricia F..

A mixed culture and a pure bacterial strain (BMV8) were isolated from a bioreactor for thiocyanate treatment. Both cultures removed 5 mM of thiocyanate from the medium in 36 hours. The mixed culture was able to tolerate concentrations up to 60 mM. The efficiency of thiocyanate degradation decreased when the cells were immobilized.

Three days of intermittent stretching after muscle disuse alters the proteins involved in force transmission in muscle fibers in weanling rats

Palavras-chave: Biodegradation; Cyanate; Immobilization; Microorganisms; Thiocyanate.

Ano: 2004

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000300012

BJMBR

Gianelo,M.C.S.; Polizzelo,J.C.; Chesca,D.; Mattiello-Sverzut,A.C..

The aim of this study was to determine the effects of intermittent passive manual stretching on various proteins involved in force transmission in skeletal muscle. Female Wistar weanling rats were randomly assigned to 5 groups: 2 control groups containing 21- and 30-day-old rats that received neither immobilization nor stretching, and 3 test groups that received 1) passive stretching over 3 days, 2) immobilization for 7 days and then passive stretching over 3 days, or 3) immobilization for 7 days. Maximal plantar flexion in the right hind limb was imposed, and the stretching protocol of 10 repetitions of 30 s stretches was applied. The soleus muscles were harvested and processed for HE and picrosirius staining; immunohistochemical analysis of collagen...

Use of Coffee Capsules as Support Material in Upflow Anaerobic Fixed Bed Reactors

Palavras-chave: Immobilization; Vimentin; Desmin; Intermittent stretching; Collagen.

Ano: 2016

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000200603

Souza,Gabriela Rezende de; Oliveira,Luiz Fernando Coutinho de; Bello,Italoema Pinheiro; Siniscalchi,Luciene Alves Batista; Fia,Ronaldo; Gandia,Rômulo Marçal.

Abstract The aim of this work is to evaluate the performance of upflow anaerobic fixed bed reactors filled with espresso coffee capsules to treat sanitary sewage. Three reactors (R1, R2 and R3) were constructed in blue PVC pipes measuring 30 cm height and 150 mm diameter and filled with coffee capsules made of aluminum and plastic. The sewage from the pre-treatment phase of the wastewater treatment plant of the Federal University of Lavras fed the system. Temperature, pH, alkalinity and volatile acids concentration, COD, TS, TVS and TSS of the influent and effluent were analyzed to evaluate the reactors performances. Statistics tests were run in the software Statistica 10. Changes occurred in the organic loading rates caused two different operating phases,...

Utilización de la κ-carragenina como soporte de inmovilización de microorganismos marinos degradadores de hidrocarburos.

Palavras-chave: Anaerobic filter; Biomass; Immobilization; Support material; Domestic sewage.

Ano: 2019

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100223

OceanDocs

Fonseca, E. L.; Núñez, R. R.; Villaverde, M. J; Barbán, O. M.; Ferrer, O. B.; Ramos, I.; Valdés, O..

Una marea negra provocada por el vertido deliberado o accidental de crudos de petróleo, aceite combustible, fangos o residuos petrolíferos y productos refinados, provoca un gran impacto sobre los ecosistemas marinos y costeros. En este trabajo, se desarrolla un producto compuesto por bacterias marinas inmovilizadas por atrapamiento en k- carragenina semirrefinada y en cuya formulación no se incluyen nutrientes. Los resultados muestran que la variante de inmovilización más ventajosa se obtuvo cuando fue empleada la κ-carragenina al 1% gelificada con una solución de KCL 1.5 M. Mediante análisis de Cromatografía Gaseosa de Alta Resolución y de espectroscopia (FTIR), se demostró la alta capacidad de degradación del bioproducto frente a petróleo del Yacimiento...

Tipo: Preprint

Palavras-chave: Immobilization; Oil; Immobilization; Http://aims.fao.org/aos/agrovoc/c_27550.

Ano: 2006

URL: http://hdl.handle.net/1834/3662

Whole cells in enantioselective reduction of benzyl acetoacetate

Ribeiro,Joyce Benzaquem; Ramos,Aline de Souza; Lopes,Raquel de Oliveira; Silva,Gabriela Veloso Vieira da; Souza,Rodrigo Octavio Mendonça Alves de.

The β-ketoester benzyl acetoacetate was enantioselectively reduced to benzyl (S)-3-hydroxybutanoate by seven microorganism species. The best result using free cells was obtained with the yeast Hansenula sp., which furnished 97% ee and 85% of conversion within 24 h. After immobilization in calcium alginate spheres, K.marxianus showed to be more stable after 2 cycles of reaction.